This study was conducted to determine the effects of row spacing (RS) and irrigation (IR) on protein content, oil content, and fatty acid composition of soybeans grown in the Harran region of Turkey. Oil content of the seed varied from 20.9 to 22.3%. Oil and protein contents were both affected by year. RS and IR affected protein and oil contents significantly (P < 0.01) in both years. RS of 70 cm had the highest protein content, followed by RS of 60, 40, and 50 cm, respectively. IR every 3rd day resulted in the highest level of protein, followed by 6th, 9th, and 12th day irrigation, respectively. The correlation coefficient (r) between protein and oil content was -0.791 in 1998 and -0.721 in 1999. RS (P < 0.01) and IR (P < 0.01) influenced oleic and linoleic acid contents significantly. Interactions of RS and IR were also found to be significant (P < 0.05) for the oleic and linoleic acid contents of soybeans.