Akademik Veri Yönetim Sistemi
Journal of Dentistry, cilt.161, 2025 (SCI-Expanded, Scopus)
Objectives: This study aimed to evaluate the effects of incorporating a hydroxyapatite-binding antimicrobial peptide (HBAMP) into a self-adhesive resin cement (SARC) on shear bond strength to enamel and dentin, surface microhardness, degree of monomer conversion, and water sorption/solubility properties. Methods: HBAMP was incorporated into a SARC at concentrations of 0.5 % and 1 % (w/w). The study groups consisted of (1) control (SARC without HBAMP), (2) SARC containing 0.5 % HBAMP (0.5 % HBAMP@SARC), and (3) SARC containing 1 % HBAMP (1 % HBAMP@SARC) Thirty-six mandibular third molars and 36 maxillary central incisors were used to evaluate shear bond strength (SBS) to enamel and dentin, respectively. For enamel SBS testing, crowns were sectioned at the cemento-enamel junction, and labial enamel surfaces were embedded in acrylic resin. For dentin SBS testing, occlusal enamel was removed and tooth surfaces were standardized using abrasive papers. Composite resin blocks (3 mm diameter x 2 mm height) were bonded to the prepared surfaces using SARC. SBS was measured using a universal testing machine. Additionally, disc-shaped specimens (6 mm diameter x 1 mm height) were fabricated for surface microhardness (Vickers), degree of monomer conversion (via FTIR analysis), and water sorption/solubility testing. Data were analyzed statistically. Results: The incorporation of HBAMP into the SARC at concentrations of 0.5 % and 1 % significantly enhanced SBS to enamel (p < 0.05). While both concentrations also numerically increased SBS to dentin, only the 1 % HBAMP group showed a significant improvement (p < 0.05). No significant changes were observed in microhardness or degree of monomer conversion at either concentration (p > 0.05). Water sorption and solubility were significantly increased in the 1 % HBAMP group (p < 0.05), whereas the 0.5 % group exhibited a significant increase in water sorption only (p < 0.05), with no significant change in solubility (p > 0.05). Conclusion: HBAMP appears to be a safe and effective additive to SARC, enhancing bonding performance, particularly to dentin, without compromising mechanical or polymerization properties. Significance: The incorporation of HBAMP into self-adhesive resin cement offers a novel strategy to enhance dentin adhesion without compromising the material's fundamental mechanical properties. By improving shear bond strength, particularly to dentin, while maintaining microhardness and monomer conversion, HBAMP-modified cement demonstrates potential for advancing the durability and reliability of indirect dental restorations. These findings provide a foundation for future development of bioactive adhesive materials with improved performance and therapeutic benefits.