A novel deep eutectic solvent microextraction method for traces tartrazine has developed for its separation and preconcentration prior to its determination by UV-Vis spectrophotometry at 430 nm. In order to obtain deep eutectic solvent, tetrabutylammonium bromide and decanoic acid were used. The important analytical parameters such as pH, deep eutectic solvent type, model solution volume, sample volume, and matrix effects are optimized. Tartrazine was quantitatively extracted in the deep eutectic solvent phase at pH 4.0. The limit of detection and preconcentration factor were found as 0.084 mg L-1 and 12.5, respectively. The validation of the procedure was performed by using addition/recovery tests. The method was applied to the determination of tartrazine content of various environmental samples.