Research Information System
1st International Conference on Natural Products for Cancer Prevention and Therapy, İstanbul, Turkey, 31 August - 02 September 2015, pp.13, (Summary Text)
Pelargonium species; members of Geraniaceae family, originate from South Africa and different species are found in distinct habitats. A medicinal plant native to the South Africa is Pelargonium sidoides have been used to treat cough, sore throat, congestion and other respiratory ailments. In Türkiye Pelargoniums are represented by two species: P.endlicherianum Fenzl. and P.quercetorum Agnew. This study was performed to investigate the cytotoxicity and GSH-Px activities of different P.endlicherianum root extracts on A549 human lung adenocarcinoma epithelial cell line. %11 Ethanol, %70 methanol extracts and the fractions ethyl acetate and butanol extracts that were obtained by the fractionation of %70 methanol extract, were used. For cytotoxicity screening Sulforhodamine B colorimetric assay and xCELLigence Real Time Cell Analysis (RTCA) were used. The cell growth was measured using ELISA reader after staining with Sulforhodamine B dye (SRB) which binds to basic amino acid residues in the trichloroacetic acid (TCA) fixed cells. The concentrations of the extracts were 0,125; 0,25; 0,5 mg/ml. %70 Methanol and ethylacetate extracts decrease the cell growth under %50. The xCELLigence system measures the impedance variations in the culture media of a 96-well microelectronic plate, and shows the changes in cell number and morphology in a real-time plot. At the same concentrations of extracts, Real Time Cell Analysis results supported the SRB results. The cells at a number 1×106 were seeded to two 48 well plate. 10 μl 1 ng/ml concentrated IL-1β was applied to one of the 48 well plate and 10 μl culture media applied to other plate. After 3 h incubation the extracts were applied at a concentration 50,100 and 200 μg/ml. Incubation 3 h with the extracts under %5 CO2 at 37°C the cell lysates were than analysed spectrophotometrically via a multifunctional microplate reader. In non–applied IL-1β groups ethyl acetate and butanol extracts increased the GPx activity. In all the concentrations ethyl acetate and butanol fractions increased the activity of GPx while 200 μg/ml %70 methanol and %11 ethanol extracts decrased in IL-1β applied group. The results reveal that the ethyl acetate and butanol fractions support the lung carcinoma cell line by increasing the GSHP-x activity response to the inflammation.