Supramolecular Solvent Liquid Phase Microextraction of Voriconazole İn Pharmaceutical and Environmental Samples With High Performance Liquid Chromatography Detection
Cumhuriyet Science Journal, vol.45, no.3, pp.490-495, 2024 (TRDizin)
- Publication Type: Article / Article
- Volume: 45 Issue: 3
- Publication Date: 2024
- Doi Number: 10.17776/csj.1455893
- Journal Name: Cumhuriyet Science Journal
- Journal Indexes: TR DİZİN (ULAKBİM)
- Page Numbers: pp.490-495
- Erciyes University Affiliated: Yes
Abstract
In this study, a new analytical method based on the supramolecular solvent liquid phase microextraction (Ss-LPME) and HPLC was developed for the analysis of voriconazole as an active drug in the class of antifungals with a wide spectrum of action at trace levels. Nano/micro sized supramolecular phase formed in 1-decanol, tetrahydrofuran and aqueous environment was used as extraction agent for separation and preconcentration of trace level of voriconazole. For the optimization of the Ss-LPME method, important analytical parameters such as the effect of sample solution pH, volume of 1-decanol, amount of THF, ultrasounic irridation time, centrifugation time and sample solution volume on the extraction efficiency were evaluated. Optimal conditions of the Ss-LPME; pH: 8, 1-decanol volume: 200 µL, THF volume: 300 µL, ultrasounic irridation time:10 min and centrifugation time: 8 min. For the developed Ss-LPME/HPLC procedure, the limit of detection (LOD), limit of quantification (LOQ) and enhancement factor (EF) were found 2.7 µg·L-1, 8.8 µg·L-1 and 36, respectively. The Ss-LPME/HPLC procedure was applied to waste water, lake water and drug samples. The fact that recovery values ranging from 100% to 108.5% were obtained for these real samples proved that this method can be used successfully in the matrix environments studied