Characterization of the RNA dependent DNA polymerase of bovine leukemia virus

DEMİRHAN I., Altanerova V., Chandra A., Gerber T., Hofmann D., Altaner C., ...More

ANTICANCER RESEARCH, vol.16, pp.2501-2505, 1996 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16
  • Publication Date: 1996
  • Journal Name: ANTICANCER RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2501-2505
  • Erciyes University Affiliated: Yes

Abstract

The reverse transcriptase - RNA dependent DNA polymerase of Bovine Leukemia Virus (BLV) was isolated and characterized. The enzyme has a molecular weight of about 80kd and the isoelectric point is 7.6. The enzyme prefers magnesium, as a divalent cation using synthetic homopolymeric template primer poly (C) oligo (dG). Monoclonal antibodies directed against reverse transcriptase of human immunodeficiency virus type I (HIV-I) did not crossreact with the isolated polymerase.