Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Zor K., Dymek K., Ortiz R., Faure A. M. , Saatci E. , Gorton L., ...Daha Fazla

FOOD CHEMISTRY, cilt.133, ss.598-603, 2012 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 133 Konu: 2
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1016/j.foodchem.2012.01.065
  • Sayfa Sayıları: ss.598-603


A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay. (C) 2012 Elsevier Ltd. All rights reserved.