Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Zor, Kinga; Dymek, Katarzyna; Ortiz, Roberto; Faure, Audrey; Saatci, EBRU; Gorton, Lo; Bardsley, Ronald; Nistor, Mihaela

doi:10.1016/j.foodchem.2012.01.065

Indirect, non-competitive amperometric immunoassay for accurate quantification of calpastatin, a meat tenderness marker, in bovine muscle

Atıf İçin Kopyala

Zor K., Dymek K., Ortiz R., Faure A. M., Saatci E., Gorton L., ...Daha Fazla

FOOD CHEMISTRY, cilt.133, sa.2, ss.598-603, 2012 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 133 Sayı: 2
Basım Tarihi: 2012
Doi Numarası: 10.1016/j.foodchem.2012.01.065
Dergi Adı: FOOD CHEMISTRY
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.598-603
Erciyes Üniversitesi Adresli: Evet

Özet

A method is described for quantification of the beef tenderness marker, calpastatin, in meat samples by amperometric detection. Using a novel bovine recombinant partial calpastatin protein as standard antigen a low detection limit of 0.2 ng/mL was achieved. The influence of the complex matrix was minimised by heat pretreatment and dilution of the samples prior to detection of calpastatin. The relative error between the direct sample measurement and standard addition methods was 5.89%, confirming the accuracy of the developed amperometric immunoassay. (C) 2012 Elsevier Ltd. All rights reserved.