The cytotoxic effects of Crystaphalmum dichotomum on A549 and HepG2 cell lines


AYCAN M. B., DOKUMACI A. H., Akşit A., Çelik İ., GÜRBÜZ P.

2nd Anticancer Agent Congress, Muğla, Turkey, 23 - 25 April 2014, pp.62

  • Publication Type: Conference Paper / Full Text
  • City: Muğla
  • Country: Turkey
  • Page Numbers: pp.62
  • Erciyes University Affiliated: Yes

Abstract

The cytotoxic effects of Crystaphalmum dichotomum on

A549 and HepG2 cell lines

Mükerrem Betül Yerer-Aycan*, Alim Hüseyin Dokumacı*,

Abdüssamed Akşit, İsmail Çelik, Perihan Gürbüz**

Erciyes University, Faculty of Pharmacy, Dept. of Pharmacology*, Pharmacognosy ** 38100 Kayseri Türkiye. E-mail: eczbetul@yahoo.com

Background / Aim

We investigated the cytotoxic effects of Crystaphalmum dichotomum on A 549 (human lung cancer) and Hep G2 ( human liver cancer) cell lines.

Materials and Methods

SRB assay

 A 549  and  Hep G2 cells were purshased from ATCC. Hep G2 and A549 cells were dispensed to plates  in complete F12K - Kaighn Medium and DMEM - Dulbecco's Modified Eagle Medium, respectively. Each mediums supplemented with fetal bovine serum (FBS) , 100 U/ml penisilin and 100 mg/ml streptomycin.. The cells were grown to % 80 confluence and treated with extracts for 24 hours: C. dichotomum (5,10,50,100,250 µg/ml) at 37 0C and % 5 CO2. Cell viability was assessed by SRB (Sulforhodamine B ) colorimetric method  for cytotoxicity screening.

Xcelligence (Real-Time Cell Analyzer) analysis

Xcelligence cell index (CI) impedance measurements were performed according to the instructions of the supplier. A549 and Hep G2 were resuspended in medium and seeded 6250 cells/100 μL. Cells were monitored every 15 min for a period of up to 120 hour by the xcelligence system.

Results

SRS assay

High dose (250 µg/ml)  C. dichotomum decreased viability around % 80 on A 549 cell lines, whereas decreased viability around % 50 on Hep G2 via SRB method.

Xcelligence results ;

 

Hep G2 cells

 

A 549 cells

Control        5 µg/ml         10 µg/ml           50 µg/ml         100 µg/ml    250µg/ml

Conclusion

C. dichotomum high dose decrease cell viability. Therefore with regard to this preliminary results, it is thought that mechanism of C. dichotomum  can be investigated by advanced experiments and might be beneficial in lung and liver  cancer theraphy.