Research Information System
2nd Anticancer Agent Congress, Muğla, Turkey, 23 - 25 April 2014, pp.62, (Full Text)
The cytotoxic effects of Crystaphalmum dichotomum on
A549 and HepG2 cell lines
Mükerrem Betül Yerer-Aycan*, Alim Hüseyin Dokumacı*,
Abdüssamed Akşit, İsmail Çelik, Perihan Gürbüz**
Erciyes University, Faculty of Pharmacy, Dept. of Pharmacology*, Pharmacognosy ** 38100 Kayseri Türkiye. E-mail: eczbetul@yahoo.com
Background / Aim
We investigated the cytotoxic effects of Crystaphalmum dichotomum on A 549 (human lung cancer) and Hep G2 ( human liver cancer) cell lines.
Materials and Methods
SRB assay
A 549 and Hep G2 cells were purshased from ATCC. Hep G2 and A549 cells were dispensed to plates in complete F12K - Kaighn Medium and DMEM - Dulbecco's Modified Eagle Medium, respectively. Each mediums supplemented with fetal bovine serum (FBS) , 100 U/ml penisilin and 100 mg/ml streptomycin.. The cells were grown to % 80 confluence and treated with extracts for 24 hours: C. dichotomum (5,10,50,100,250 µg/ml) at 37 0C and % 5 CO2. Cell viability was assessed by SRB (Sulforhodamine B ) colorimetric method for cytotoxicity screening.
Xcelligence (Real-Time Cell Analyzer) analysis
Xcelligence cell index (CI) impedance measurements were performed according to the instructions of the supplier. A549 and Hep G2 were resuspended in medium and seeded 6250 cells/100 μL. Cells were monitored every 15 min for a period of up to 120 hour by the xcelligence system.
Results
SRS assay
High dose (250 µg/ml) C. dichotomum decreased viability around % 80 on A 549 cell lines, whereas decreased viability around % 50 on Hep G2 via SRB method.
Xcelligence results ;
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Hep G2 cells
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A 549 cells |
Control 5 µg/ml 10 µg/ml 50 µg/ml 100 µg/ml 250µg/ml
Conclusion
C. dichotomum high dose decrease cell viability. Therefore with regard to this preliminary results, it is thought that mechanism of C. dichotomum can be investigated by advanced experiments and might be beneficial in lung and liver cancer theraphy.