Detection of Escherichia coli O157:H7 using immunomagnetic separation and mPCR in Turkish foods of animal origin


Ertas N. , Gonulalan Z. , YILDIRIM Y. , Karadal F., ABAY S. , AL S.

LETTERS IN APPLIED MICROBIOLOGY, vol.57, no.4, pp.373-379, 2013 (Journal Indexed in SCI) identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 4
  • Publication Date: 2013
  • Doi Number: 10.1111/lam.12124
  • Title of Journal : LETTERS IN APPLIED MICROBIOLOGY
  • Page Numbers: pp.373-379

Abstract

The present study was conducted to investigate the presence of Escherichia coli O157:H7 in food samples of animal origin and to detect its virulence genes by immunomagnetic separation technique and multiplex PCR (mPCR). A total of 500 samples (consisting of diced meat, minced meat, burger, raw cow's milk and raw cow's milk cheese) were analysed. Escherichia coli O157:H7 was detected in 5 (1%) of 500 analysed samples including two diced meat, one minced meat and two raw-milk cheese. None of the burger samples tested contained E.coli O157:H7. Three isolates obtained from minced and diced meat were found to carry stx(1), stx(2), hlyA and eaeA genes whereas two isolates from raw-milk cheese were found to harbour the stx(1), eaeA and hlyA genes. The results of this study suggest that raw meat and raw-milk cheese tested could pose public health problems in consumers with regard to their virulence factors.