Protein Profiling of Indegnous Common Bean Through SDS-PAGE

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Sher K., Nisar M., Subhan M., Hazrat A., Muhammad A., Hancı F., ...Daha Fazla

Bioscience Research, cilt.17, sa.2, ss.688-698, 2020 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 2
  • Basım Tarihi: 2020
  • Dergi Adı: Bioscience Research
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.688-698
  • Anahtar Kelimeler: Phaseolus vulgaris, seeds, Genetic diversity, Genetic association, SDS-PAGE, GENETIC DIVERSITY
  • Erciyes Üniversitesi Adresli: Evet

Özet

This activity was aimed to investigate the proteomic diversity of common bean landraces collected from the different ecological zones of Malakand division, KP, Pakistan. The genetic diversity was assessed through seed storage protein profile using SDS-PAGE of 117 landraces collected from different ecological zones of Malakand division, KP, Pakistan. The protein summaries were confirmed via SDSPAGE. The electrophoregram produced ten bands ranging from 180 to 10 kDa with Molecular Weight Marker. The electrophoregram exhibited two zones RI and RII. The R-I, contained six bands ranging in size from 180 to 34kDa and RII is comprised of 4 bands ranging in size from 33 to 10kDa.The average Genetic Index for R-I was 17%, while for R-II, it was 27%. Among all bands, B-4 and B-5 was found to have the greatest genetic diversity (90.59% and 88.88%, respectively). The dendrogram based on R-I and R-II zones sorted all the landmarks into 13 and 10 clusters respectively, showed high level of GD within 117 landraces. High degree of Variation in protein band profile and multiple clustering validate that in future Pakistani bean landraces will provide an abundant chance for obtaining genetically strong and stable genotype with high valued production to breeders.