Evaluation of organic-inorganic hybrid nanoflower's enzymatic activity in the presence of different metal ions and organic solvents


Altinkaynak C., Gulmez C., Atakisi O., ÖZDEMİR N.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, vol.164, pp.162-171, 2020 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 164
  • Publication Date: 2020
  • Doi Number: 10.1016/j.ijbiomac.2020.07.118
  • Title of Journal : INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • Page Numbers: pp.162-171
  • Keywords: Lipase-inorganic hybrid nanoflowers, Metal ions, Organic solvents, Enzymatic activities, ENHANCED CATALYTIC-ACTIVITY, INDUSTRIAL APPLICATIONS, LIPASE, PERFORMANCE, PURIFICATION

Abstract

In the present study, lipase-inorganic hybrid nanoflowers (L-hNFs) have indicated to possess several novel characteristics that are significant in terms of industrial worth. In detail, we reported the production of lipase-inorganic hybrid nanoflowers in different parameters and evaluated synthesized nanoflowers' enzymatic activity in the presence of different concentrations of metal ions and organic solvents. The optimum pHs of free enzyme, L-hNFs (0.02, 0.05, and 0.1) and L-hNFs (0.03) were pH 8, 7 and 6, respectively. L-hNFs (0.05) displayed the highest enzymatic activity in high alkaline pH values (10, 11, and 12) compared to both free enzyme and other L-hNFs. While the free enzyme showed no activity at pH 12, L-hNFs (0.05) maintained 57% of their activity. The optimum temperatures of the free lipase and L-hNFs were 30 degrees C and 40 degrees C, respectively. Free lipase activity decreased with temperature while L-hNFs had higher enzyme activity at 40 degrees C and 50 degrees C. Even in the case of increasing incubation and organic solvent and metallic ion concentration in which free lipase cannot completely maintain its activity, L-hNFs were able to protect their activity. L-hNFs exhibited excellent thermal and pH stability and high resistance to metallic ions and organic solvents at various concentrations for 3 h, 6 h, and overnight. This new lipase-inorganic hybrid nanobiocatalyst may be promising in many industrial processes such as chemical, biochemical, pharmaceutical, and biotechnological ones. (C) 2020 Elsevier B.V. All rights reserved.