Antioxidative effects of cysteamine, hyaluronan and fetuin on post-thaw semen quality, DNA integrity and oxidative stress parameters in the Brown Swiss bull


SARIÖZKAN S. , TUNCER P. B. , Buyukleblebici S., Bucak M. N. , Canturk F. , EKEN A.

ANDROLOGIA, vol.47, no.2, pp.138-147, 2015 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1111/and.12236
  • Title of Journal : ANDROLOGIA
  • Page Numbers: pp.138-147
  • Keywords: Antioxidant activities, cryopreservation, DNA damage, lipid peroxidation, sperm parameters, OXYGEN SPECIES GENERATION, IN-VITRO, HUMAN-SPERMATOZOA, SPERM MOTILITY, LIPID-PEROXIDATION, SEMINAL PLASMA, GLUTATHIONE-PEROXIDASE, STRAND BREAKS, BASE DAMAGE, CRYOPRESERVATION

Abstract

The aim of this study was to compare the effectiveness of antioxidants including cysteamine (2.5, 7.5mm), hyaluronan (0.25, 1mgml(-1)) and fetuin (5, 10mgml(-1)) in the freezing of Brown Swiss bull semen. The best percentages of CASA motilities were achieved with 10mgml(-1) of fetuin and 2.5mm of cysteamine. For sperm morphology, 10mgml(-1) of fetuin and 2.5mm of cysteamine had better protective effects (P<0.001). The results of hypo-osmotic swelling test showed that the percentage values of membrane integrity in all the groups, excluding that supplemented with 5mgml(-1) of fetuin, were higher than those of the control group (P<0.001). Results obtained for the DNA damage of sperm cells demonstrated that 0.25mgml(-1) of hyaluronan, and 2.5 and 7.5mm of cysteamine led to lower rates of spermatozoa with damaged DNA, compared with the control group (P<0.001). The maintenance of superoxide dismutase and glutathione peroxidase antioxidant activities following freeze-thawing with 2.5 and 7.5mm of cysteamine and 10mgml(-1) of fetuin was demonstrated to be at a higher level in comparison with the control group (P<0.001). Malondialdehyde formation was found to be lower in the groups supplemented with 0.25mgml(-1) of hyaluronan and 7.5mm of cysteamine after the freeze-thawing process (P<0.001).