Application of the pseudo-plaque assay for detection and titration of Crimean-Congo hemorrhagic fever virus

Berber E., Canakoglu N., Yoruk M. D., Tonbak S., Aktas M., ERTEK M., ...Daha Fazla

JOURNAL OF VIROLOGICAL METHODS, cilt.187, sa.1, ss.26-31, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 187 Sayı: 1
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1016/j.jviromet.2012.07.025
  • Dergi Adı: JOURNAL OF VIROLOGICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.26-31
  • Anahtar Kelimeler: Titration, Detection, Crimean-Congo hemorrhagic fever, Turkey-Kelkit06, Pseudo-plaque assay, NEUTRALIZATION TEST, QUANTITATION, TURKEY, ANTIBODIES, DIAGNOSIS, CELLS
  • Erciyes Üniversitesi Adresli: Evet

Özet

A pseudo-plaque assay was developed for detection and quantitation of Crimean-Congo hemorrhagic fever virus Turkey-Kelkit06. Enzyme-catalyzed color development of infected cells probed with anti-Crimean-Congo hemorrhagic fever virus antibodies was used for determining the titer of Crimean-Congo hemorrhagic fever Turkey-Kelkit06 and for its detection in samples from persons infected with the Crimean-Congo hemorrhagic fever virus. The pseudo-plaque assay accuracy was confirmed by comparing pseudo-plaque assay titers with fluorescent immunofocus assay and focus formation assay titers using three stocks of virus. No significant difference in virus titers of Crimean-Congo hemorrhagic fever Turkey-Kelkit06 among the three methods was observed. The pseudo-plaque assay is more sensitive than the fluorescent immunofocus assay for detecting the virus in primary isolates of Crimean-Congo hemorrhagic fever virus collected from humans, but no difference in sensitivity between the two methods was observed in the cell-adapted strain of Crimean-Congo hemorrhagic fever Turkey-Kelkit06. The pseudo-plaque assay is suitable for titration of Crimean-Congo hemorrhagic fever Turkey-Kelkit06, which does not develop plaques, suggesting it may also be suitable for the detection of other viruses. (C) 2012 Elsevier B.V. All rights reserved.