A total of 83 watermelon varieties registered by Ministry of Food, and Agriculture and Livestock of Turkey were used in this study. They were characterized both with 76 morphological traits according to UPOV and molecular SRAP markers. Genetic relationships were determined using NTSYS2.1 statistical software based correlation matrix and unweighted pair group method arithmetic average (UPGMA). The 76 morphological traits were used for principal coordinate analysis. The four main groups included 4, 3, 9 and 67 varieties, respectively. The 83 watermelon varieties were genotyped by 7 SRAP primer combinations. A total of 32 bands were produced and 11 of them were polymorphic. The watermelon cultivars used in this study showed a low level of polymorphism among commercial cultivars for SRAP markers. The results indicate the power of the morphological characters to discriminate among the watermelon varieties, and the usefulness in selecting the parental genotypes used in breeding programs.