Akademik Veri Yönetim Sistemi
Erciyes Üniversitesi Fen Bilimleri Enstitüsü Dergisi, cilt.37, sa.2, ss.348-355, 2021 (Hakemli Dergi)
In this study, we aimed to determine the toxic effect of increasing doses of
Bendiocarb, an insecticide from the carbamate group, on human blood cells in vitro via
by Comet test, determination of total antioxidant capacity, antioxidant enzyme activity
and malondialdehyde (MDA) level. Leukocyte isolation was performed from blood
samples taken from six healthy male individuals who did not smoke and use alcohol and
were not exposed to any chemicals in the working environment, and then their
leukocytes exposed to increasing doses of Bendiocarb (20, 40, 80, 160 µg / mL). Comet
test, iron reduction antioxidant capacity (FRAP), 2-2'azinobis 3-ethylbenzothiazoline-6-
sulfonic acid (ABTS/TEAC) analysis with superoxide dismutase (SOD), catalase (CAT),
glutathione peroxidase (GPx) and MDA were evaluated. At the end of the study, it was
determined that antioxidant enzyme levels decreased significantly due to dose increase,
whereas MDA levels increased significantly in all doses (p<0.05). According to FRAP and
TEAC methods, the antioxidant capacity of blood cells decreased significantly in
increasing doses of Bendiocarb compared to the control group (p<0.05). In the Comet
test, DNA tail percentage, length and moment were increased significantly in increasing
doses of Bendiocarb and DNA damage was detected (p<0.05). This study is a preliminary
study on this subject, and it has been shown in our study that the uncontrolled use of
Bendiocarb pesticide in nature will have harmful effects on the environment and living
things. We believe that the research will contribute to the literature and shed light on
future comprehensive studies.