A new definition about the relationship of intercellular fluid in the brain with the mandibular and parotid lymph nodes

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Nur İ. H., Keles H., Unlukal N., Solmaz M., Erdoğan E., Perez W.

MICROSCOPY RESEARCH AND TECHNIQUE, cilt.85, sa.1, ss.220-232, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1002/jemt.23898
  • Dergi Adı: MICROSCOPY RESEARCH AND TECHNIQUE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.220-232
  • Anahtar Kelimeler: brain drainage, glymphatic system, intracellular fluid, lymph nodes, Qdot 800, CEREBRAL AMYLOID ANGIOPATHY, CENTRAL-NERVOUS-SYSTEM, DEEP CERVICAL LYMPH, CEREBROSPINAL-FLUID, INTERSTITIAL FLUID, SUBARACHNOID SPACE, DRAINAGE PATHWAYS, ALZHEIMER-DISEASE, DIFFERENT REGIONS, VASCULAR SYSTEM
  • Erciyes Üniversitesi Adresli: Evet

Özet

This study was carried out to reveal the relationship of the brain with both the mandibular lymph node (MLN) and parotid lymph node (PLN) by the hyperspectral fluorescence imaging techniques of Qdot 800 (QD) nanoparticles using in vivo. This relationship of the brain with both lymph nodes offers the preliminary morphological definition of lymphatic drainage. QD was injected into the left parietal brain lobe of each rat at a depth of 2.50 mm. In 65% of the rats that were imaged in vivo, signals were received first from the right MLN and PLN, and then from the left MLN and PLN. In contrast, in two female rats, the first signal was received from the right PLN. There was no difference between the female and male rats overall. The most noteworthy finding of this study was that the tracer injected into the left parietal lobe reached the right mandibular and parotid lymph nodules earlier. This result indicates a different and unknown pathway in the brain that communicates with the lymph nodes. Moreover, this study shows that these lymph nodes pathways can be used in the treatment of diseases such as brain trauma, cerebral edema, and Alzheimer's disease (AD).