Deficiency of Boron (B) is widespread in the many parts of region of Turkey. So, the effects of boron and plant growth promoting bacteria (Bio-B) on the fruit yield, antioxidant enzyme activity and plant freeze injury of strawberry cv. Fern were investigated under field conditions between 2013 and 2014. The experimental plot was a completely randomized design with 4 replicates. Control and Bio-B were used as fertilizer agent in the experiment. Bio-B fertilizer was applied in three methods as soil, foliar and soil + foliar application methods to strawberry plants. Data through 2 years showed that the use of Bio-B significantly increased fruit yield, antioxidant enzyme activity and decreased freeze injury of strawberry leaf. Soil + foliar applications of Bio-B fertilizer increased to fruit yield compared to the control by 55.91 %. However, foliar application of Bio-B fertilizer increased to catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) enzyme activity compared with the control treatment 41.86 %, 48.99 %, and 26.59 %, respectively and decreased freeze injury of strawberry leaves 27.41 %. Overall, the results of this study suggest that Bio-B fertilizer application have the potential to increase the yield, antioxidant enzyme activity and decreased freeze injury of strawberry plants under field conditions.