Gold Nanoparticle-Coated Surfaces as Advanced Interfaces for Enhanced Raman Detection of Biological Samples
6th Life Sciences Congress, Kayseri, Türkiye, 13 - 14 Şubat 2026, ss.64, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Kayseri
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.64
- Erciyes Üniversitesi Adresli: Evet
Özet
Raman spectroscopy is a label-free analytical technique capable of revealing detailed molecular information from biological samples, yet its practical sensitivity is often limited by inherently weak scattering signals. Nanostructured metallic surfaces, particularly those incorporating noble metal nanoparticles, are known to influence light–matter interactions and may improve spectral detection. In this study, we investigated the use of gold nanoparticle (AuNP)-coated glass substrates as potential signal-enhancing platforms for Raman spectroscopic analysis of biological materials. Glass slides were coated with gold nanoparticles to produce a nanostructured metallic interface designed to promote stronger optical interactions at the sample surface. As a biological test system, cultured cells were seeded onto AuNP-coated substrates and uncoated glass controls. Raman spectra were acquired under identical experimental conditions to evaluate differences in signal intensity, spectral clarity, and peak definition. Samples measured on AuNP-coated surfaces exhibited noticeable variations in spectral intensity and improved definition of characteristic Raman bands compared to measurements on standard glass. Differences were observed in regions associated with major biomolecular constituents such as proteins, lipids, and nucleic acids. While the precise enhancement mechanism was not directly characterized, the presence of gold nanoparticles appears to influence spectral signal behavior, potentially through nanoscale electromagnetic interactions at the cell–substrate interface. Overall, these findings suggest that gold nanoparticle-coated substrates may contribute to improved Raman signal detection from biological samples. The stability and biocompatibility of gold-based nanostructured surfaces further support their potential use in developing sensitive, label-free spectroscopic platforms for biological and biomedical applications.