The Effects of MBL1 Gene Polymorphism on Subclinical Mastitis in Holstein Cows

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Aksel E. G. , Akcay A. , Arslan K. , Sohel M. H. , Güngör G. , Akyüz B.

KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, vol.27, no.3, pp.389-395, 2021 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 3
  • Publication Date: 2021
  • Doi Number: 10.9775/kvfd.2021.25714
  • Page Numbers: pp.389-395


This study aims to investigate the effects of three single nucleotide (SNPs) polymorphisms of the MBL1 gene on subclinical mastitis for Holstein cows. For this study, a total of 151 Holstein cows were selected in their third lactation. The subclinical mastitis conditions were determined using the California Mastitis Test (CMT). Genotyping was carried out with the PCR-RFLP method. The results revealed that the wild-type allele frequencies were 0.72 (G allele), 0.40 (G allele), and 0.37 (T allele) for 1252 G>A, 2534 G>A, and 2569 T>C SNPs, respectively. Among the investigated SNPs, only the 1252 G>A SNP was not found in Hardy-Weinberg equilibrium. The effects of SNPs on subclinical mastitis were modeled using multiple logistic regression analysis. The established model can identify cows with subclinical mastitis with a separation efficiency of 62.3%. It was thought that 1252 G>A and 2534 G>A SNPs may affect subclinical mastitis rates.