Multivariate statistical design optimization for ultrasonic-assisted restricted access supramolecular solvent-based liquid phase microextraction of quercetin in food samples

MEMON Z. M., YILMAZ E., SOYLAK M.

JOURNAL OF THE IRANIAN CHEMICAL SOCIETY, cilt.14, sa.12, ss.2521-2528, 2017 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 12
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1007/s13738-017-1187-9
  • Dergi Adı: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2521-2528
  • Anahtar Kelimeler: Quercetin, Restricted access supramolecular solvents, Liquid phase microextraction, Multivariate statistical design, Nutrition, Food samples, DOEHLERT MATRIX, SPECTROPHOTOMETRIC DETERMINATION, PRECONCENTRATION SYSTEM, ZINC DETERMINATION, HUMAN URINE, EXTRACTION, ABSORPTION, PERFORMANCE, FLAVONOIDS, WATER
  • Erciyes Üniversitesi Adresli: Evet

Özet

In this method, quercetin as a flavonoid has been extracted, preconcentrated and determined by using ultrasonic-assisted restricted access supramolecular solvent-based liquid phase microextraction method in food samples. The quercetin concentration in extraction phase was determinated by UV-visible spectrophotometer which has microsampling cuvette. Multivariate statistical design approach was used to optimize the analytical variables including the pH, ratio of restricted access solvent components, volume of restricted access solvent, ultrasonication and centrifugation times. The analytical performance values of the developed method including limit of detection, limit of quantification, preconcentration factor and relative standard deviation (10 replicates of 10-5 M of quercetin solution) were found as 2.98, 9.93 mu g L-1, 30 and 6.3%, respectively. The method provides important advantages such as use of minimum volume of organic solvents, simple and economical operation and easy optimization via multivariate statistical design approach. The developed procedure was validated with four food samples, and acceptable recoveries (87-104%) were achieved.