Akademik Veri Yönetim Sistemi
Current Trends in Natural Sciences, Pitesti, Romanya, 18 - 20 Nisan 2019, ss.30-31
Knowing the impact of circadian clock
control on lymphocytes, undergoing DNA double strand break (DSBs) and repair,
can help athletes to decide the best time for exercise. To assess the impact of
strenuous exercise at sunrise and sunset on DSBs and repair in immune cells of amateur
athletes. The study was conducted on May-June 2017 as quasi-experimental
interrupted time series design. Samples from 12 healthy volunteer amateur athletes
(at the age range of 19-25) at sunrise were taken Just before (0h) and 2, 24,
48 and 72h after exercise for analysis. That group underwent strenuous exercise
at sunset at 15 days interval with the same design and analysis as in sunrise. AKLIDES cell damage system was used for determining DSBs
(phosphorylated H2AX) and repair (53BP1) in lymphocytes using indirect
immunofluorescence. At
sunrise the DNA DSBs assays revealed a remarkable increase (median
foci-mean, 0.092) just after 2h and returned to baseline at the next 24h
(median foci-mean, 0.027). Whereas at sunset, DSBs assay showed a significant
increase (median foci mean, 2.373) just after the 2ndh, and only
returned to baseline levels (median foci mean, 0.251) after
48h. The dsDNA repair assay at sunrise demonstrated a significant increase
(median foci-mean, 0.067) immediately after the 2nd h
and returned to normal levels
within 24h. At sunset a striking increase was observed in the dsDNA
repair assay (median foci mean, 0.089) during 48h, and returned to baseline
only after 72h. Considering the sensitivity of immune system cells to
internal biological clocks, amateur athletes must synchronize their training
time with the circadian clock recovery period in order to keep their
immunological health at the best level. Since the DSBs are at the lowest and
the repair activities are at the peak level in the morning, sunrise appears to be the best time to perform
strenuous exercises while insuring a better immune wellness.