In this study, the relationship between plasma vWF antigen level and polymorphism occurring due to the leucine (L) to proline (P) substitution at position 2380 in exon 42 in von Willebrand factor gene (VWF) in dogs was aimed to investigate. The present study was performed on 161 dogs of various breeds and ages (95 male, 66 female) referred to Faculty of Veterinary Clinics at Erciyes University between January 2014 and 2015. Blood samples were collected in EDTA and Na citrate tubes after clinical examination of the dogs. PT, APTT, TT, fibrinogen, D-dimer, thrombocyte, vWF antigen (vWF: Ag) levels and VWF were determined from the blood samples. Genotypes were examined using PCR and restriction endonucleas enzymes. In the laboratory examination, 34 (21.1%) of the cases were positive and 127 (78.9%) of the cases were negative with concern to von Willebrand. Prevalence of the disease in different dog breeds that included to the present study were Golden Retreiver (n=35) 34.3%, Kangal (n=45) 11.1%, German Shepherd (n=11) 36.4%, Labrador Retriever (n=7) 42.9%, English Cocker Spaniel (n=2) 100%, English Pointer (n=8) 25.0%, mix breed (n=8) 33.3%, Husky (n=7) 12.5%, Malinois (n=1) 100%, Dogo Argentino (n=2) 50.0%, Samoyed (n=1) 100% and some other breeds were 0%. The genotype of VWF was not statistically significant in both positive and negative dogs with concern to vWF: Ag values (P=0.675). When comparison were made in terms of proportional distribution in positive and negative dogs, statistical importance were not observed between genotype and the disease ratio (P=0.969). In conclusion, relationship between VWF and vWF antigen level were not determined in dogs. According to our knowledge; this study is the first repot on VWF and vWF: Ag relationship therefore believed to be important.