Cytotoxic Activity of Opopanax hispidus (Friv.) Griseb.: Characterization of a New Chalcone-type Compound
Chemistry and Biodiversity, vol.22, no.11, 2025 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 22 Issue: 11
- Publication Date: 2025
- Doi Number: 10.1002/cbdv.202501077
- Journal Name: Chemistry and Biodiversity
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
- Keywords: Apiaceae, chalcone, cytotoxic activity, lung cancer, Opopanax hispidus
- Erciyes University Affiliated: Yes
Abstract
The present study aimed to investigate cytotoxic compounds of Opopanax hispidus (Friv.) Griseb. through in vitro, in silico, and phytochemical analyses. The cytotoxic activity of O. hispidus was evaluated against four different cancer cell lines – lung (A549), breast (MCF-7), liver (HepG2), and cervix (HeLa) – as well as one healthy cell line (Beas-2B), using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay. n-Hexane and dichloromethane sub-extracts with the IC50 values of 9.77 ± 0.57 and 7.10 ± 0.78 mg/mL on A549, respectively, were fractionated through chromatographic techniques, and the structures of the isolated compounds were elucidated using various spectroscopic methods. A novel compound, 2',6'-dihydroxy-4'-methoxy-3''-(ɤ,ɤ-dimethylallyl)-4''-hydroxy β-hydroxydihydrochalcone (3), along with three known coumarins – umbelliferone 6-carboxylic acid (1), umbelliferone (2), and nodakenetin (4) – were isolated from the active fractions. Compound 3, which possesses a chalcone structure, exhibited notable cytotoxicity against lung cancer cells with an IC50 value of 13.72 ± 0.80 µM.