Research Information System
3rd International Congress of Medical and Health Sciences Studies, Ankara, Turkey, 13 - 14 December 2024, pp.93
damaging the brain tissue, which can subsequently affect the entire body system. TBI is a significant health issue that affects people of all ages and socioeconomic groups and has a high mortality and morbidity rate. However, the molecular mechanisms that affect the vital functions and treatment process after TBI have yet to be fully understood. Alkaline Phosphatase (Alpl) and Fibroblast Growth Factor (bFGF) genes, expressed in tissues and organs that play a crucial role in regulating vital functions, may influence the clinical phenotypes developed after trauma. In this study, we aimed to investigate the expression levels of Alpl ve bFGF in the traumatic mice models. Materials and Methods: The study was conducted at Erciyes University Genome and Stem Cell Research Center (GENKOK). A total of 30 animals were randomly divided into three groups, with equal numbers of males and females in each group. Mild TBI (mTBI) was induced in mice utilizing the Marmarau trauma model. After applying the mTBI model, Alpl and bFGF expression levels in the acute and chronic phases were determined via Real-Time PCR in liver, kidney, bone, and muscle tissues. Results: Alpl and bFGF gene expressions in the acute phase after TBI were increased significantly. In the kidney, while Alpl and bFGF expression levels increased in the acute phase in females, bFGF significantly decreased in males. bFGF expression levels significantly increased in males in the chronic phase in the muscle tissue. Our study showed significant differences between sexes in response to mTBI. Conclusion: Our study investigated the role of Alpl and bFGF genes in peripheral tissues in acute and chronic phases after mTBI for the first time in the literature. The data obtained will guide understanding the secondary events and the consequences of the disease in mTBI and taking the necessary treatment and measures.