Improving Raman Signal Sensitivity Using Silver Nanoparticle-Coated Substrates for Biological Analysis
6th Life Sciences Congress, Kayseri, Türkiye, 13 - 14 Şubat 2026, ss.61, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Kayseri
- Basıldığı Ülke: Türkiye
- Sayfa Sayıları: ss.61
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Erciyes Üniversitesi Adresli: Evet
Özet
Raman spectroscopy is a powerful, label-free analytical technique that provides detailed molecular information from biological samples. However, its sensitivity is often limited due to the inherently weak Raman scattering signal. Nanostructured metallic surfaces, particularly those incorporating silver nanoparticles (AgNPs), interact strongly with incident light and can enhance spectroscopic signals through localized electromagnetic effects. In this study, we evaluated the potential of silver nanoparticle-coated glass substrates to improve Raman signal detection from biological specimens. Glass slides were coated with silver nanoparticles to create a nanostructured surface that could facilitate stronger light–matter interactions. As a biological test system, cultured cells were seeded onto both AgNP-coated substrates and uncoated control glass slides. Raman spectra were collected under identical acquisition parameters to assess differences in signal intensity and spectral quality. Samples measured on AgNP-coated surfaces showed increased spectral intensity and sharper peaks than those on standard glass. Signal differences were particularly noticeable in spectral regions associated with major biomolecular components, including proteins, lipids, and nucleic acids. While the enhancement mechanism was not directly characterized in this study, the observed spectral amplification suggests that silver nanoparticle coatings may promote stronger electromagnetic interactions at the sample–substrate interface. These findings suggest that silver nanoparticle-coated substrates have the potential to function as signal-enhancing interfaces for Raman spectroscopic analysis of biological samples. The use of such nanostructured surfaces may improve spectral sensitivity and signal clarity, thereby supporting the broader application of label-free vibrational spectroscopy in biological and biomedical studies.