Unbiased analysis of senescence associated secretory phenotype (SASP) to identify common components following different genotoxic stresses


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ÖZCAN S. , Alessio N., ACAR M. C. , Mert E., Ömerli F., PELUSO G., ...Daha Fazla

AGING-US, cilt.8, ss.1316-1329, 2016 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 8 Konu: 7
  • Basım Tarihi: 2016
  • Doi Numarası: 10.18632/aging.100971
  • Dergi Adı: AGING-US
  • Sayfa Sayıları: ss.1316-1329

Özet

Senescent cells secrete senescence-associated secretory phenotype (SASP) proteins to carry out several functions, such as sensitizing surrounding cells to senesce; immunomodulation; impairing or fostering cancer growth; and promoting tissue development. Identifying secreted factors that achieve such tasks is a challenging issue since the profile of secreted proteins depends on genotoxic stress and cell type. Currently, researchers are trying to identify common markers for SASP. The present investigation compared the secretome composition of five different senescent phenotypes in two different cell types: bone marrow and adipose mesenchymal stromal cells (MSC). We induced MSC senescence by oxidative stress, doxorubicin treatment, X-ray irradiation, and replicative exhaustion. We took advantage of LC-MS/MS proteome identification and subsequent gene ontology (GO) evaluation to perform an unbiased analysis (hypothesis free manner) of senescent secretomes. GO analysis allowed us to distribute SASP components into four classes: extracellular matrix/cytoskeleton/cell junctions; metabolic processes; ox-redox factors; and regulators of gene expression.