Simple and sensitive determination of vitamin A and E in the milk and egg yolk samples by using dispersive solid phase extraction with newly synthesized polymeric material


Köseoğlu K., ULUSOY H. İ., YILMAZ E., SOYLAK M.

JOURNAL OF FOOD COMPOSITION AND ANALYSIS, vol.90, 2020 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 90
  • Publication Date: 2020
  • Doi Number: 10.1016/j.jfca.2020.103482
  • Journal Name: JOURNAL OF FOOD COMPOSITION AND ANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Analytical Abstracts, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Food Science & Technology Abstracts, Veterinary Science Database
  • Keywords: Vitamin A, Vitamin E, Dispersive solid phase extraction, Polymer based materials, Milk and egg yolks, FAT-SOLUBLE VITAMINS, PRESSURIZED LIQUID EXTRACTION, ALPHA-TOCOPHEROL, BETA-CAROTENE, HPLC, CHROMATOGRAPHY, MICROEXTRACTION, WATER, TOCOTRIENOLS, SUPPLEMENTS
  • Erciyes University Affiliated: Yes

Abstract

A simple and sensitive determination method for trace amounts of vitamins A and E has been developed based on dispersive solid phase extraction (DSPE) and HPLC-DAD analysis in this study. In the proposed method, the vitamin molecules were retained on a newly synthesized solid phase material consisting of tetracycline-grafted polyacrylamide polymer (PAA-T). The method was applied by the conventional batch method and desorption of retained vitamins from PAA-T surface were carried out with acetonitrile. Then, the samples were filtered through a 0.45 mu m filter, transferred to HPLC vials. The analysis of the samples was carried out using a C18 column at a flow rate of 1 mL min(-1) with isocratic elution consisting of methanol (30 %) and acetonitrile (70 %). For vitamin A (retinol) and vitamin E(alpha-tocopherol) respectively; determination wavelengths: 324 and 292 nm, linear ranges: 20-700 ng mL(-1) and 50-1800 ng mL(-1), limit of detections (LOD): 5.71 ng mL(-1) and 14.28 ng mL(-1), relative standard deviations(RSD) for 5 repetitive measurements: 3.25 % and 2.85 %. For application of the developed method, recovery experiments were carried out and quantitative values were obtained in milk and egg yolks.