In living organisms heterogenous air pollutants are considered as a major source of DNA damage. In this study, we aimed to describe the DNA changes in Pseudevernia furfuracea (L.) Zopf samples exposed to pollution at various sites in Kayseri by RAPD (random amplified polymorphic DNA) analysis in order to reveal the pattern of genetic variation influenced by the environmental pollution. The study area is characterized by the presence of numerous industrial activities, such as steel works, glassworks, shipbuilding, and metallurgical, mechanical, chemical and food industries. Control samples were collected from C, at forest (Sivas) and exposed to pollution in dry and wet seasons in the province of Kayseri located in Central Anatolia. Results indicated that heterogeneous pollutants might have contributed to the changes in the band patterns obtained by RAPD analysis, reflecting the occurrence of DNA damage in the control samples.