The present research was undertaken to evaluate antioxidant activities and enzyme inhibitory potentials of two extracts from two parts of Mandragora autumnalis. The antioxidant properties were evaluated by using different methods [ABTS (2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid)), DPPH (1,1 diphenyl-2-picrylhydrazyl radical), CUPRAC (cupric ion reducing antioxidant capacity), and FRAP (ferric reducing antioxidant power assays)]. Enzyme inhibitory potentials were tested against cholinesterase (AChE and BChE), tyrosinase, a-amylase and a-glucosidase. For phytochemical composition, fatty acids, total phenolics and flavonoid content were investigated. Generally F-Met exhibited the strongest antioxidant effect with the highest level of phenolics. Total phenolic and flavonoid content were determined as 26.10-46.92 mgGAE/g abd 3.60-26.11 mgRE/g, respectively. a- linolenic acid was found to be major fatty acids in the studied oils (33.81 % for flowers and 39.15 % for leaves). The study suggested that M. autumnalis could be valuable as a source of natural agents in food and pharmaceutical applications.