Ensuring Clonal Fidelity and Quality Assurance in Woody Plant Micropropagation
Next-Generation In Vitro Strategies in Woody Plant Cloning, Springer Nature, ss.385-409, 2026
- Yayın Türü: Kitapta Bölüm / Araştırma Kitabı
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/978-3-032-28071-8_15
- Yayınevi: Springer Nature
- Sayfa Sayıları: ss.385-409
- Anahtar Kelimeler: Clonal uniformity, Genetic stability, Molecular markers, Somaclonal variation
- Erciyes Üniversitesi Adresli: Evet
Özet
Traditional propagation methods for woody plant species, such as cuttings, grafting, and seed systems, often fail to meet global demands for consistent, disease-free, high-quality planting materials. In vitro clonal propagation has become a key biotechnology for large-scale production and conservation of high-value woody germplasm. However, a significant challenge remains in maintaining clonal fidelity, as hormonal imbalances, oxidative stress, extended subculturing, or dedifferentiation complicate somaclonal variation. This chapter reviews the biological foundations and technical steps of woody plant micropropagation, covering totipotency, explant physiology, media, and plant growth regulators (PGRs), before exploring sources of variation and the importance of producing true-to-type clones. It then discusses quality assurance techniques, including morphological evaluation, cytological assessment, and molecular markers, and presents selected case studies across major woody genera. Results show that axillary-bud and meristem-based systems generally maintain high genetic stability, whereas callus-based or prolonged-culture methods increase the risk of variation. This chapter concludes that implementing a multilayered monitoring system, utilizing optimized culture protocols, limiting regeneration cycles, and applying routine molecular screening is crucial for ensuring the genetic and phenotypic integrity of woody clones, thereby supporting both commercial cultivation and conservation efforts.