Phytochemical composition and cytotoxic activity of four Cousinia Species against human hepatocellular carcinoma (HepG2) cell line
Phytomedicine Plus, cilt.1, sa.4, 2021 (Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 1 Sayı: 4
- Basım Tarihi: 2021
- Doi Numarası: 10.1016/j.phyplu.2021.100068
- Dergi Adı: Phytomedicine Plus
- Derginin Tarandığı İndeksler: Scopus
- Anahtar Kelimeler: Asteraceae, Cousinia, Cytotoxicity, HepG2, LC-MS/MS
- Erciyes Üniversitesi Adresli: Evet
Özet
Background: Cousinia Cass. is one of the most diverse genus of Asteraceae family. According to previous studies some Cousinia species showed significant cytotoxic activity. Purpose: In this study, we aimed to investigate the cytotoxic activity and phytochemical profile of four Cousinia species (C.davisiana Hub.-Mor., C.foliosa Boiss. & Balansa, C.ramosissima DC, C.stenocephala Boiss). Methods: The cytotoxic activity was investigated with SRB method against HepG2 (human hepatocellular carcinoma) cell line. The quantitative and qualitative determination of compounds were carried out by LC-MS/MS. Results: In a result, C. davisiana methanol extract (CD) and ethyl acetate sub-extract (CDE) showed more cytotoxic activity with 150 and 89 µg/ml IC50 values, respectively. The mass spectrums revealed the presence of 15 compounds in CD methanol extract and 9 compounds in active CDE sub-extract. According to quantitative analyses the highest contents of all compounds were detected in CD extract. Conclusion: Moreover, this study on inhibitory activity of CD extract and CDE sub-extract suggests detailed investigation for cytotoxic activity.