Green Synthesis of Zinc Oxide Nanoparticles from Malus domestica, Tilia cordata, and its Biological Activities

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Güler N., Aslan H. G., Karaköse E., Güllü M.

Journal of New Results in Science, cilt.14, sa.2, ss.166-180, 2025 (TRDizin)

• Yayın Türü: Makale / Tam Makale
• Cilt numarası: 14 Sayı: 2
• Basım Tarihi: 2025
• Doi Numarası: 10.54187/jnrs.1698629
• Dergi Adı: Journal of New Results in Science
• Derginin Tarandığı İndeksler: EBSCO Legal Source, TR DİZİN (ULAKBİM)
• Sayfa Sayıları: ss.166-180
• Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
• Erciyes Üniversitesi Adresli: Evet

Özet

This study aimed to evaluate the anticancer and antibacterial drug status of biomass,

including zinc oxide (ZnO) nanoparticles (NPs) synthesized from two plant extracts according to the

results of Topoisomerase-I enzyme inhibition, DNA cleavage, DNA binding, and antibacterial

activity tests. The Malus domestica (apple) and Tilia cordata (linden) plants were dried and

powdered, and their extracts were prepared in pure water. X-Ray Diffraction (XRD), Scanning

Electron Microscopy (SEM), and Fourier Transform Infrared (FTIR) analyses were applied for the

characterization of the light-yellow powder ZnO NPs. Additionally, Topoisomerase-I enzyme

inhibition, DNA cleavage, and DNA binding analyses were performed by agarose gel electrophoresis

method. Powder ZnO NPs (biomass) were analyzed by the agar well diffusion method by using two

gram-positive and two gram-negative bacteria. According to the XRD analysis results, the peaks

obtained show that there is no foreign matter or secondary diffraction peak other than the hexagonal

ZnO structure. It was observed that ZnO NPs from Tilia cordata extract are effective against

Klebsiella pneumoniae. ZnO NPs from Malus domestica extract have the potential to be an effective

human Topoisomerase-I enzyme inhibitor. There was poor DNA cleavage activity in all samples. In

ZnO NPs from linden extract, there was an interaction at a 1mg/mL concentration of CT-DNA.