Tailoring a MOF-on-MOF (ZIF-8-on-NH2-MIL-53(Al)) Hybrid Composite for Micro Solid Phase Extraction of Furosemide in artificial biological fluids prior to LC-q-TOF/MS detection

GÜMÜŞ Z. P., SOYLAK M.

Advances in Sample Preparation, cilt.13, 2025 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.sampre.2025.100157
  • Dergi Adı: Advances in Sample Preparation
  • Derginin Tarandığı İndeksler: Scopus
  • Anahtar Kelimeler: Doping, Furosemide, Mass Spectrometry, Metal Organic Frameworks, MOF-on-MOF, Solid Phase Extraction, Zeolitic Imidazolate Frameworks
  • Erciyes Üniversitesi Adresli: Evet

Özet

Furosemide, a powerful diuretic that increases urine excretion, is used to treat many diseases. However, due to its abuse in sport, it is considered a doping substance. As a result, it has been banned by the World Anti-Doping Agency (WADA) and included in the group of diuretics and masking agents. Given the clinical significance of furosemide and its dependable application in athletics, this research employed the "MOF-on-MOF" hybrid material composite to leverage the synergistic effects of various components for detecting this drug in biological samples. After characterization of the ZIF-8-on-NH2-MIL-53(Al) composite by FT-IR, XRD, TGA, XPS, BET and SEM-EDX techniques, the µ-SPE procedure was optimized using liquid chromatography-time of flight mass spectrometry (LC-q-TOF/MS) based on the recovery values. The optimum conditions were determined as pH 6, adsorbent amount of 20 mg, and total adsorption-desorption time of 7 min. The recovery values of the solid-phase extraction method for simulated body fluids samples under optimum conditions were between 77.5 % and 93.4 %. The µ-SPE technique was effectively utilized on physiological samples using the ZIF-8-on-NH2-MIL-53(Al) composite under optimal conditions. This study's findings will inform future research into the design of 'MOF-on-MOF' hybrids for various applications.