Carcinogenic bromate (BrO-3) can be present in drinking water as a result of its formation from bromide (Br-) during ozonation. A fixed bed column reactor filled with elementel sulfur and limestone was operated for about 6 months under autotrophic and mixotrophic (autotrophic+heterotrophic) conditions at 30oC. The reactor was operated at the hydraulic retention time (HRT) ranging from 16.5 to 10.1 h at autotrophic conditions. Under mixotrophic conditions, 45 mgL-1 NO3-N was removed completely at C:N ratio (mg CH3OH:mg NO3-N) between 0.55 and 1.66 at HRT of 10.1 h. The average effluent pH was 7.8 and the sulfate concentration was lower than the Environmental Protection Agency (EPA) limits at the mixotrophic stages. Efficient simultaneous bromate and nitrate removal was achieved at feed concentrations of 100-500 µgL-1 bromate and 45 mgL-1 nitrate under autotrophic and mixotrophic conditions. Effluent bromide measurements indicated that bromate was completely reduced without accumulation of by-products.