MOUSE MODEL OF GENERATIONAL ACQUIRED PSORIASIS INDUCED BY IMIQUIMOD
14TH INTERNATIONAL İSTANBUL SCIENTIFIC RESEARCH CONGRESS , İstanbul, Turkey, 26 - 27 December 2024, pp.343, (Summary Text)
- Publication Type: Conference Paper / Summary Text
- City: İstanbul
- Country: Turkey
- Page Numbers: pp.343
- Erciyes University Affiliated: Yes
Abstract
Psoriasis is a common chronic inflammatory skin disease characterized by excessive proliferation of keratinocytes, which develops with the influence of genetic and environmental factors, familial transmission has been detected but no associated gene or mutation has been identified, and it is known that the disease is triggered by environmental factors. We believe that diseases such as psoriasis, in which genetic inheritance has been detected but no associated gene or mutation has been identified, are generationally acquired genetic diseases that are inherited generationally with the influence of environmental factors. Based on this idea, in this study, we aimed to create a transgenerationally acquired psoriasis mouse model. Based on this aim, generations were completed by applying imiquimod for 3 generations in our study. Each generation was treated with imiquimod starting from the 6th week and the generations were completed. Sham groups, treated with Vaseline, and control groups with no treatment were also formed. After mating, mice were sacrificed, and skin biopsies, sperm, and blood samples were collected to analyze telomere lengths and TERRA levels. According to our findings, in the F0 generation psoriasis group, telomere lengths were shortened in blood and lesional, and non-lesional skin, while no significant difference was detected in DNA:RNA hybrid TERRA and total TERRA expression levels. In the F1 generation psoriasis group, increased telomere lengths were detected in sperm, lesional, and non-lesional skin, with no significant differences in TERRA expression levels. In the F2 generation, shortened telomere lengths were observed in blood, sperm, lesional, and non-lesional skin, alongside increased DNA:RNA hybrid TERRA expression levels