Development and validation of a liquid chromatography–tandem mass spectrometry method of a panel of commonly prescribed benzodiazepines in urine samples
SEPARATION SCIENCE PLUS, vol.0, no.08, 2023 (ESCI, Scopus)
- Publication Type: Article / Article
- Volume: 0 Issue: 08
- Publication Date: 2023
- Doi Number: 10.1002/sscp.202300036
- Journal Name: SEPARATION SCIENCE PLUS
- Journal Indexes: Emerging Sources Citation Index (ESCI), Scopus
- Keywords: benzodiazepine, chromatography, confirmation, drug testing, urine
- Erciyes University Affiliated: Yes
Abstract
Benzodiazepines are one of the most prescribed for therapeutic purposesand the most abused drugs. Therefore, liquid chromatography–tandem massspectrometry–based methods that analyze several benzodiazepines simultane-ously are required. In this study, we developed a simple, full validated in-housemethod in a single-triple quadrupole platform SCIEX QTRAP 4500 system forthe simultaneous detection and quantification of the 10 most prescribed ben-zodiazepines within a 10-min run time. The linear range was 12.5–500 ng/mL.All limits of detection were below 4.03 ng/mL and limits of quantitation below12.22ng/mL,exceptclobazam(7.27and22.04ng/mL,respectively).Boththelimitof detection and quantitation were lowest for diazepam (0.95 and 2.89 ng/mL,respectively). The method was evaluated for accuracy, precision, carry-over,recovery, stability, and matrix effects, and all validation studies met internation-ally acceptable criteria. The applicability of the method was proven by analyzingauthentic urine samples, internal, and third-party external quality samples. Thisprecise,accurate,shortertotalruntimeandeaseofurinesampleanalysismethodis applicable in detecting most prescribed benzodiazepines and their metaboliteseven in glucuronide forms.