Akademik Veri Yönetim Sistemi
Journal of Molecular Structure, cilt.1370, 2026 (SCI-Expanded, Scopus)
Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide, with a significantly higher incidence in males (male-to-female ratio of 2.4:1). Current HCC screening methods mainly rely on serological and radiographic tests; however, their imaging sensitivity varies depending on the operator and equipment, ranging between 34% and 84%. Therefore, the development of specific biomarkers and highly sensitive detection techniques for HCC remains an urgent need. Recent studies have demonstrated that nanostructure-based electrodes significantly improve sensitivity in electrochemical sensing. In this study, we aimed to design a biosensor probe capable of accurate, sensitive, and stable detection of HCC. For this purpose, AFP antibodies, selected metal ions, and magnetic nanoparticles (MNPs) were utilized to construct an AFP antibody–metal phosphate hybrid nanoflower (hNF)-based biosensor probe immobilized on a carbon electrode. Various characterization techniques such as SEM, TEM, FT-IR, XRD were employed during synthesis and conjugation steps, and the detection limit and linear range of the fabricated probe were determined. The effects of different pH values, temperatures, and storage periods on the stability of the synthesized MNP–hNFs were also investigated. The advantageous resistance mechanisms of MNP–hNFs on the probe surface (immune response and weak conductivity) contributed to interfacial charge-transfer modulation that amplified the measurable electrochemical response for target molecule detection. As a biological carrier, the MNP–hNF structure provided a large number of active sites, enhanced molecular capture, and provided an increased interfacial surface availability relative to conventional nanoparticle systems, offering significant benefits for electrochemical biosensor applications.