COMPARISON OF CYTOMEGALOVIRUS (CMV) ANTIGENEMIA AND CMV-DNA HYBRID CAPTURE ASSAYS USED IN THE DIAGNOSIS OF CMV INFECTION

GOKAHMETOGLU S., ÇETİN M.

İNFEKSİYON DERGİSİ, vol.17, no.4, pp.385-388, 2003 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 17 Issue: 4
  • Publication Date: 2003
  • Journal Name: İNFEKSİYON DERGİSİ
  • Journal Indexes: TR DİZİN (ULAKBİM)
  • Page Numbers: pp.385-388
  • Erciyes University Affiliated: Yes

Abstract

2003, Cilt 17, Sayı 4, Sayfa(lar) 385-388
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COMPARISON OF CYTOMEGALOVIRUS (CMV) ANTIGENEMIA AND CMV-DNA HYBRID CAPTURE ASSAYS USED IN THE DIAGNOSIS OF CMV INFECTION
Selma GÖKAHMETOĞLU1, Melek İNCİ1, Yusuf ÖZBAL1, Mustafa ÇETİN2, Tamer GÜNEŞ3
1Erciyes Üniversitesi Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Kayseri
2Erciyes Üniversitesi Tıp Fakültesi, Hematoloji Bilim Dalı, Kayseri
3Erciyes Üniversitesi Tıp Fakültesi, Çocuk Sağlığı ve Hastalıkları Anabilim Dalı, Kayseri
Keywords: Cytomegalovirus (CMV), CMV antigenemia assay, CMV-DNA hybrid capture assay, diagnosis
Cytomegalovirus (CMV) is a major cause of severe infection in transplant recipients and also causes congenital infection in newborns. Detection of CMV pp65 antigen and CMV-DNA is important for the diagnosis of CMV disease. The aim of this study was to compare CMV antigenemia and CMV-DNA assays used in the diagnosis of CMV infection. Totally of 49 blood samples from 24 patients suspected of CMV disease were included in the study. Twelve of the patients were newborns and 12 were bone marrow and renal transplant recipients. The blood samples of transplant recipients were collected in months 1-3 following transplantation. Cytomegalovirus pp65 antigen was detected by indirect immunofluorescence technique (CINAkit, Argene-Biosoft, France), and CMV-DNA by hybrid capture assay (Digene, USA). Cytomegalovirus antigen and CMV-DNA were negative in 30 (61%) of the samples. Both assays were found positive in five. Nine samples were antigenemia positive, but CMV-DNA negative. Five samples were CMV-DNA positive, but antigenemia negative. No significant difference was found between the two methods by χ2 test. It is concluded that CMV antigenemia and CMV-DNA assays should be performed together for the diagnosis of CMV infection.