The aim of this study is to investigate electromagnetic shielding and comfort properties of single jersey fabrics produced from hybrid yarns containing metal wire. For this purpose, nine different hybrid yarns were produced from the combination of cotton yarns (Ne 50, Ne 60, and Ne 70) and stainless steel (SS) wires (18 micron, 35 micron, and 50 micron) by means of hollow spindle covering system. Hybrid yarns and 100 % cotton yarns used in the production of hybrid yarns were used to produce knitted fabric on the sample circular knitting machine. When the shielding effectiveness (SE) values of the sample fabrics were examined, it was found that the SE value of knitted fabrics containing SS wire were higher than that of without SS wires. It was also found that as the SS wire thickness of knitted fabric increases, thermal conductivity and thermal absorptivity values increase, but air permeability and vertical wicking values decrease.