A green, novel and effective ultrasound assisted-deep eutectic solvent (DES) extraction (UA-DES-E) procedure was developed for extraction of iron from sheep, bovine and chicken liver samples. The analytical parameters including type and composition DES, volume of DES, ultrasonication time and ratio of sample to DES were optimized by using 50 mg of the NIST SRM 1577b bovine liver certified reference material. The limit of detection (LOD) and the limit of quantification (LOQ) were found as 0.026 mL(-1) and 0.085 mu g mL(-1), respectively. The relative standard deviation (RSD) as a result of 7 replicates of 50 mg of certified reference material was 1.4%. The accuracy of proposed method was checked by the addition/recovery tests to NIST SRM 1577b bovine liver and a sheep liver. The extraction method was applied to extraction of iron from bovine, sheep and chicken liver samples retail from markets at Kayseri, Turkey with satisfactory results (recoveries higher than 95%). (C) 2014 Elsevier B.V. All rights reserved.