Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits
MEAT SCIENCE, cilt.94, sa.4, ss.474-479, 2013 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 94 Sayı: 4
- Basım Tarihi: 2013
- Doi Numarası: 10.1016/j.meatsci.2013.04.015
- Dergi Adı: MEAT SCIENCE
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.474-479
- Anahtar Kelimeler: HNF1A, Single nucleotide polymorphism, mRNA and protein expression, Methylation, Meat quality, Pig
- Erciyes Üniversitesi Adresli: Evet
Özet
The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F-2 Duroc x Pietrain (DuPi, n = 313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n = 110) population. HNF1A mRNA and protein expressions were higher (p < 0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p > 0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect. (C) 2013 Elsevier Ltd. All rights reserved.
The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH24H, meat percentage and muscle area in the F2 Duroc × Pietrain (DuPi, n=313) and with pH24L, fat area and backfat thickness in the Pietrain (Pi, n=110) population. HNF1A mRNA and protein expressions were higher (p < 0.05) in animals with the low post-mortem muscle pH24L. The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p > 0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect.