The two novel preconcentration and separation methods based on adsorption onto Amberlite XAD-1180 and Amberlite XAD-16 polymeric resins for spectrophotometric determination of sunset yellow dye were developed. The parameters, affecting the quantitative recovery, including pH, sample and eluent flow rates, eluent type, sample volume, were investigated and optimised. The interference effects of some cations, anions and widely used dye were also studied. At the optimum conditions, detection limits of the methods were found as 2.0 and 1.6gL(-1) for Amberlite XAD-1180 and Amberlite XAD-16 resins, respectively. Linear dynamic ranges of the methods were obtained in the range of 0.2-50.0 and 0.2-20.0gmL(-1) for Amberlite XAD-1180 and Amberlite XAD-16 resins, respectively. The relative standard deviations were below than 7% and 5% for Amberlite XAD-1180 and Amberlite XAD-16 resins, respectively. The determination of dye was performed at 483.0nm using spectrophotometry. Validations of the methods were performed comparatively with determination of the sunset yellow content of some foodstuffs and pharmaceuticals.