Akademik Veri Yönetim Sistemi
International Congress on Biological and Health Sciences (ICBH), Afyonkarahisar, Türkiye, 26 Şubat - 28 Temmuz 2021, ss.67, (Özet Bildiri)
Herpes simplex virus-1 (HSV-1) is leading cause of ocular impairment in human across the
world. Loss of vision is due to inflammatory reaction caused by pro-inflammatory CD4 T cells.
HSV-1 infection lead to stromal keratitis (SK) and angiogenesis in mouse model. Viral induced
immunopathological finding can be reversed by promoting anti-inflammatory immune cells and
this can be done by manipulating the metabolism of cell. In this report we investigate that
manipulating amino acid can reduce the viral induced immunopathological lesion. Amino acid
metabolism mainly glutamine was blocked by injecting intraperitoneally 6-Diazo-5-oxo-Lnorleucine (DON) in ocular HSV-1 infected mice. On day 15th post infection mice were
euthanized and flow cytometry analysis was done. In-vitro generation of splenocyte from RAG-/-
mice was also done in presence or absence of DON. We found that CD4 Th1, Th17 T cells,
neutrophils and macrophages was less in corneal of treated group and percentage of Treg get
enhanced. In treated group levels of CD4 Th1, neutrophils and macrophages was reduced at
trigeminal ganglion (TG). At drainage lymph node (DLN) level there was decrease level of CD4
Th1, Th17 T cells, neutrophils and macrophages and at the same time ratio of T regulatory (Treg)
to Th1 and Th17 cells was enhanced. Similar results were observed in spleen also. During invitro differentiation of splenocyte, addition of DON reduces Th17 cell and enhanced Treg cells
level. Glutamine metabolism can also effect the latency stage of HSV-1. Use of DON help in
reducing the reactivation of virus from latently infected TG. So, we can conclude that blocking
amino acid metabolism can reduce immunopathological lesion caused by virus and at same time
it also helps to maintain the latency of virus.