2nd Anticancer Agent Congress, Muğla, Turkey, 23 - 25 April 2014, pp.100
Investigation of cytotoxic effects of Pelargonium endlicherianum extracts and related phenolic compounds: Esculetin and Scopoletin.
Alim Hüseyin Dokumacı 1, Gökhan Ünal1, Gökçe Şeker Toprak2, Mükerrem Betül Yerer-Aycan1
1 Erciyes University, Faculty of Pharmacy, Pharmacology, 38100 Kayseri Türkiye(ahuseyindokumaci@gmail.com)
2 Erciyes University, Faculty of Pharmacy, Pharmacognosy, 38100 Kayseri Türkiye
Background / Aim
Esculetin and scopoletin are phenolic compounds that are found in various natural plant products including Pelargonium sp. and inhibit proliferation in several types of human cancer cells. The aim of this study was to investigate cytotoxicity of esculetin, scopoletin and several Pelargonium endlicherianum extracts in non-small human lung cancer cell line (A 549 cells)
Materials and Methods
Trading A 549 cells were purshased from ATCC. Approximately 25000 cells were dispensed to plates in complete F12K Kaighn medium supplemented with medium with fetal bovine serum (FBS) , 100 U/ml penisilin and 100 mg/ml streptomycin at 37 0C and % 5 CO2. The cells were grown to % 80 confluence and treated with the following substances and extracts for 24 hours: esculetin(84 mM, 168 mM ,336 mM); scopoletin (25 mM,50 mM,100 mM); Pelargonium endlicherianum %11 ethanol and % 70 methanol extracts and butanol, and ethyl acetate fractions (0,125, 0,25 , 0,5 mg/ml). Cell viability was assessed by MTT (3-4,5- dimethylthiazolyl -2-2,5-dipenthlytetrazolium bromide) method.
Results
Esculetin and %11 Pelargonium endlicherianum ethanol extract decreased the cell viability around %20-25% whereas, scopoletin could only decrease the cell viability 12% at a dose of 50mM. 70% methanol extract could not decrease the cell viability but even increased the viability at a dose of 0.25mg/ml. Butanol and ethyl acetate fractions of 70% methanol extract decreased the cell viability but they were not as effective as esculetin and %11 metanol extract.
Conclusion
We have demonsrated that esculetin and %11 Pelargonium endlicherianum ethanol extract decrease the viability of A549 cells. Therefore with regard to this preliminary results, it is thought that esculetin and Pelargonium endlicheriaunum might be beneficial in lung cancer theraphy.