Effect of hemoglobin, triglyceride, and urea in different concentrations on compatibility between methods used in HbA1c measurement

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MUHTAROĞLU S., BARLAK KETİ D., SARAÇOĞLU H.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, cilt.47, sa.6, ss.727-734, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 47 Sayı: 6
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1515/tjb-2021-0166
  • Dergi Adı: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, Food Science & Technology Abstracts, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.727-734
  • Anahtar Kelimeler: boronate affinity chromatography, hemoglobin A1c, immunoassay, boronat afinite kromatografisi, hemoglobin A1c, immunoassay
  • Erciyes Üniversitesi Adresli: Evet

Özet

Objectives The variability between methods is seen in the existence of hemoglobin (Hb), triglyceride (TG), blood urea nitrogen (BUN) and glucose in the specimens, which can affect the measurement. Therefore, this study aimed to compare boronate affinity chromatography and turbidimetric inhibition immunoassay methods for HbA1c levels in samples containing different levels of Hb, TG, BUN and glucose. Methods In this study, a total of 412 consecutive samples were used for method comparison. The samples were divided into groups according to HbA1c, TG, BUN, Hb, and glucose levels. Clinically significant changes for HbA1c are defined as >= 0.5%. Results The mean relative difference as % was determined as 1.4% by the Bland-Altman plot of Trinity against Roche. The Passing-Bablok analysis indicated good agreement (r=0.985) between two methods. When compared the group with and without a clinically significant difference, we found differences only between glucose levels (p=0.003). Conclusions High glucose levels may cause a difference between the methods. Therefore, we suggest that a method's performance and properties should be considered prior to clinical usage.