Akademik Veri Yönetim Sistemi
Experimental and Applied Medical Science, cilt.1, sa.1, ss.17-22, 2020 (Hakemli Dergi)
Diabetes mellitus causes structural and functional impairment of the system in the organism by
affecting various organs and structures. In this study, we aimed to examine the changes in
female rat heart tissue histologically by creating experimental diabetes. 16 female adult rats
were used in the study. Rats were randomly divided into two groups as control and 3–month
diabetes. The diabetes group was formed from subjects with blood glucose levels above
250mg/dl 72 hours after 40 mg / kg streptozotocin administration. At the end of the experiment,
the heart tissues of the subjects were removed and taken into formaldehyde solution. To
examine the histological structure, haematoxylin–eosin, and immunohistochemically neuronal
nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) were stained. Heart
tissue sections belonging to the control group had histologically normal appearance. In the
diabetes group heart tissue sections, vacuolization in some cells and eosinophilic increase in
the cytoplasm of some cells were observed. The nNOS and iNOS immunoreactivity was
observed to be decreased in the diabetes group compared to the control group, but the decrease
was not statistically significant. As a chronic disease, DM causes histological damage to the
heart tissue. The resulting damage causes a decrease in nNOS and iNOS expression. It is
important to maintain NOS enzyme levels to protect tissue from the harmful effects of diabetes
and ensure normal physiological conditions.