Histological Examination of Rat Heart Tissue with Chronic Diabetes

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Karabulut D. , Akin A. T. , Kaymak E., Öztürk E. , Sayan M.

Experimental and Applied Medical Science, cilt.1, no.3, ss.17-22, 2020 (Hakemli Üniversite Dergisi)

  • Cilt numarası: 1 Konu: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.46871/0.2020.2
  • Dergi Adı: Experimental and Applied Medical Science
  • Sayfa Sayıları: ss.17-22


Diabetes mellitus causes structural and functional impairment of the system in the organism by affecting various organs and structures. In this study, we aimed to examine the changes in female rat heart tissue histologically by creating experimental diabetes. 16 female adult rats were used in the study. Rats were randomly divided into two groups as control and 3–month diabetes. The diabetes group was formed from subjects with blood glucose levels above 250mg/dl 72 hours after 40 mg / kg streptozotocin administration. At the end of the experiment, the heart tissues of the subjects were removed and taken into formaldehyde solution. To examine the histological structure, haematoxylin–eosin, and immunohistochemically neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) were stained. Heart tissue sections belonging to the control group had histologically normal appearance. In the diabetes group heart tissue sections, vacuolization in some cells and eosinophilic increase in the cytoplasm of some cells were observed. The nNOS and iNOS immunoreactivity was observed to be decreased in the diabetes group compared to the control group, but the decrease was not statistically significant. As a chronic disease, DM causes histological damage to the heart tissue. The resulting damage causes a decrease in nNOS and iNOS expression. It is important to maintain NOS enzyme levels to protect tissue from the harmful effects of diabetes and ensure normal physiological conditions.