The effect of variety and growing year on oil, protein and fatty acid composition of sesame seeds was studied. Five sesame cultivars, Muganli-57, Golmarmara, Tan-99, Orhangazi-99, Kepsut-99 and pure lines 27224 and 27206 were investigated. The oil content of sesame samples varied from 38.65 to 51.79%. The variety of Tan-99 grown in 2004 had the maximum oil value. Oil and protein content of sesame varieties grown in 2003 and 2004 years changed insignificantly (p > 0.05). The protein content of the variety of Orhangazi was the highest among other varieties. The correlation coefficient between protein and oil content was -0.372 averaged over 2003 and 2004. Oleic and linoleic acid contents were significantly affected by variety (p < 0.05). In 2003, all varieties had higher linoleic and lower oleic acid levels. The correlation coefficient 'r' between oleic acid and linoleic acid content were -0.957 for sesame varieties grown in 2003 and 2004. In general, the variety appeared to have an influence on seed composition of sesame.