Joint Meeting of Anatomical Societies, Turkey, 1 - 04 May 2011, vol.5, pp.168, (Full Text)
PP-352
Determining the heavy metal levels of malleus and
incus by optical emission spectroscopy (ICP-OES)
Ocal H*, Unur E*, Leblebici Z**, Susar H*
Department of Anatomy, Medical Faculty, Erciyes University, Kayseri,
Turkey*; Department of Biology, Faculty of Science, Erciyes University,
Kayseri, Turkey****
There are three mobile auditory ossicles (malleus, incus,
stapes) between the ear membrane in middle ear and inner ear.
We used 10 malleus and 10 incus in our study. We obtained
these bones from bone collection and cadaver those used for
education of Erciyes University in Faculty of Medicine in
Anatomy department. We determined the heavy metal content
of the ossicles (ferrum, zinc, cadmium, copper, nickel, lead,
manganese) by optical emission spectroscopy (ICP-OES).
Heavy metal analysis processes have been carried out in the
Ecology Laboratuary of Erciyes University in Science Faculty
in Biology Department by the Liberty ICP-OES Sequential
device called Varian. 0.5 grams of powdered samples were
brought ossicles taken by adding 10 ml 65% HNO3 CEMMarsh
5(CEM Corporation Mathews) was dissolved in
S168
Joint Meeting of Anatomical Societies, 19-22 May 2011, Bursa, Turkey
Anatomy
2011; 5 Suppl
microwave sample-solving device. We drew calibration charts
for each element. We read each sample for three times.
According to the heavy metal analysis processes, these heavy
metal contents have been determined in malleus as below; ferrum
0.3060-0.2987 μg g-1, zinc 0.2503-0.1143 μg g-1, cadmium
0.0028-0.0007 μg g-1, lead 0.0483-0.0125 μg g-1, copper
0.0334-0.0092 μg g-1, manganese 0.0121-0.0214 μg g-1, nickel
0.0129-0.0045 μg g-1. These heavy metal contents have been
determined in incus as below; ferrum 0.1902-0.1216 μg g-1,
zinc 0.2572-0.1214 μg g-1, cadmium 0.0021-0.0006 μg g-1,
lead 0.0385-0.0061 μg g-1, copper 0.0273-0.0053 μg g-1, manganese
0.0043-0.0045 μg g-1, nickel 0.0129-0.0045 μg g-
1.Other research results are much higher at levels than ours.
And this situation indicates that there are no heavy metal dirtiness
in the malleus and incus that we made analysis.