The use of a newly synthesized chelating resin with an o-aminophenol - type functional groups and Amberlite XAD-1180 - type supporting material for separation/ preconcentration of trace metal ions from various water samples was described. Amberlite XAD-1180-o-aminophenol chelating resin (XAD-o-AP) was synthesized using Amberlite XAD-1180 resin as solid support and o-aminophenol as the chelating ligand. The determination of Cd(II), Co(II), Cu(II), Ni(II), and Pb(II) ions was made by flame atomic absorption spectrometry (FAAS). Studying with model solutions for the optimization of the method was based on the measurement of recovery values (between 92-106%) of the analyte elements. Some analytical parameters such as pH (6.0), volume of the sample (similar to 250 mL), effect of matrix ions, flow rates of the sample (2.5 mL min(-1)) and elution solutions (2.5 mL min(-1)), concentration, type and volume of the eluent (4 mol L-1 HNO3, 20 mL) were investigated. The detection limit (3s/b, n = 20) and the relative standard deviation (n = 7) of the method were found to be in the range 0.9-4.3 mu g L-1 and 4.4-5.5%, respectively. The proposed method was successfully applied to the real samples such as wastewater, boiler feeding water and a certified reference material (Estuarine water, LGC 6016).