Akademik Veri Yönetim Sistemi
Plant Cell, Tissue and Organ Culture, cilt.162, sa.3, 2025 (SCI-Expanded, Scopus)
Nanoparticles have proven effective in enhancing regeneration, morphological development, morpho physiology, and biochemical parameters of plantlets produced in vitro. This research aimed to examine the effects of silver nanoparticles (Ag-NPs) compared to silver nitrate (AgNO3) on shoot organogenesis in the regeneration of quinoa (Chenopodium quinoa Willd.). To establish an efficient regeneration system the culture medium was then supplemented with AgNO3 and Ag-NPs at a range of concentration, including 0, 2, 4, 6, and 8 mg L−1 of three types of explants (first node, middle node, and apical node). Ag-NPs yielded higher values than AgNO3 across all measured parameters, including shoot length, callus count, leaf number, and shoot weight. A concentration of 6 mg L−1 produced the optimal results for these parameters, while 8 mg L−1 led to reduced growth, indicating a potential toxicity threshold. Apical node explants showed superior regeneration capacity across treatments. Biochemical analyses revealed increased antioxidant enzyme activity and malondialdehyde content under Ag treatments, suggesting oxidative stress, while hydrogen peroxide levels decreased, indicating enhanced reactive oxygen species scavenging at higher concentrations (4–8 mg L−1). Our result showed the Ag treatments enhanced the uptake of phosphorus, potassium, and magnesium, while high concentrations resulted in decreased sodium and calcium levels, suggesting potential toxicity and impaired ion transport. Principal component analysis was employed to explore relationships among factors associated with tissue culture, revealing a strong association between high AgNO3 concentrations, explant position, and shoot development. This study found that Ag-NPs significantly influenced the growth parameters and other traits of the plantlets. Furthermore, our results suggest that the efficiency of quinoa tissue culture can be enhanced by increasing the application of Ag in the form of nanoparticles. In conclusion, this work underscores the potential of using Ag-NPs in quinoa tissue culture.